Fig 1: CRC Secretome Drives Matrix Metalloproteinase Signaling and Collagen DepositionCCSF exposure changes collagen degradation and deposition behaviors for both pericytes and fibroblasts.(A–C) SW480 conditioned media significantly increases MMPs two and nine expressions by fibroblasts. All CRC media upregulate MMP13 expression, with HCT116 media inducing the largest change.(D–F) Pericyte MMP2 expression is significantly downregulated with exposure to CCSFs, and additionally correlates with CRC cell line aggression.Pericyte MMP13 is underexpressed with CCSF exposure, and MMP9 shows no clear trend relative to media type.(G and H) HCT116 conditioned media significantly increases the quantity of collagen deposition by fibroblasts; pericytes follow the same trend but have no significant changes. Significance: ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. Data are represented as mean ± SEM n ≥ 3.
Supplier Page from Abcam for Human MMP13 ELISA Kit